892 research outputs found
Measuring Temperature Gradients over Nanometer Length Scales
When a quantum dot is subjected to a thermal gradient, the temperature of
electrons entering the dot can be determined from the dot's thermocurrent if
the conductance spectrum and background temperature are known. We demonstrate
this technique by measuring the temperature difference across a 15 nm quantum
dot embedded in a nanowire. This technique can be used when the dot's energy
states are separated by many kT and will enable future quantitative
investigations of electron-phonon interaction, nonlinear thermoelectric
effects, and the effciency of thermoelectric energy conversion in quantum dots.Comment: 6 pages, 5 figure
Core-shell nanoparticle arrays double the strength of steel
Manipulating structure, defects and composition of a material at the atomic scale for enhancing its physical or mechanical properties is referred to as nanostructuring. Here, by combining advanced microscopy techniques, we unveil how formation of highly regular nano-arrays of nanoparticles doubles the strength of an Fe-based alloy, doped with Ti, Mo, and V, from 500 MPa to 1 GPa, upon prolonged heat treatment. The nanoparticles form at moving heterophase interfaces during cooling from the high-temperature face-centered cubic austenite to the body-centered cubic ferrite phase. We observe MoC and TiC nanoparticles at early precipitation stages as well as core-shell nanoparticles with a Ti-C rich core and a Mo-V rich shell at later precipitation stages. The core-shell structure hampers particle coarsening, enhancing the material's strength. Designing such highly organized metallic core-shell nanoparticle arrays provides a new pathway for developing a wide range of stable nano-architectured engineering metallic alloys with drastically enhanced properties. ?The Author(s) 2017.1116Ysciescopu
Theory of biopolymer stretching at high forces
We provide a unified theory for the high force elasticity of biopolymers
solely in terms of the persistence length, , and the monomer spacing,
. When the force f>\fh \sim k_BT\xi_p/a^2 the biopolymers behave as Freely
Jointed Chains (FJCs) while in the range \fl \sim k_BT/\xi_p < f < \fh the
Worm-like Chain (WLC) is a better model. We show that can be estimated
from the force extension curve (FEC) at the extension
(normalized by the contour length of the biopolymer). After validating the
theory using simulations, we provide a quantitative analysis of the FECs for a
diverse set of biopolymers (dsDNA, ssRNA, ssDNA, polysaccharides, and
unstructured PEVK domain of titin) for . The success of a specific
polymer model (FJC or WLC) to describe the FEC of a given biopolymer is
naturally explained by the theory. Only by probing the response of biopolymers
over a wide range of forces can the -dependent elasticity be fully
described.Comment: 20 pages, 4 figure
Analysis of and workarounds for element reversal for a finite element-based algorithm for warping triangular and tetrahedral meshes
We consider an algorithm called FEMWARP for warping triangular and
tetrahedral finite element meshes that computes the warping using the finite
element method itself. The algorithm takes as input a two- or three-dimensional
domain defined by a boundary mesh (segments in one dimension or triangles in
two dimensions) that has a volume mesh (triangles in two dimensions or
tetrahedra in three dimensions) in its interior. It also takes as input a
prescribed movement of the boundary mesh. It computes as output updated
positions of the vertices of the volume mesh. The first step of the algorithm
is to determine from the initial mesh a set of local weights for each interior
vertex that describes each interior vertex in terms of the positions of its
neighbors. These weights are computed using a finite element stiffness matrix.
After a boundary transformation is applied, a linear system of equations based
upon the weights is solved to determine the final positions of the interior
vertices. The FEMWARP algorithm has been considered in the previous literature
(e.g., in a 2001 paper by Baker). FEMWARP has been succesful in computing
deformed meshes for certain applications. However, sometimes FEMWARP reverses
elements; this is our main concern in this paper. We analyze the causes for
this undesirable behavior and propose several techniques to make the method
more robust against reversals. The most successful of the proposed methods
includes combining FEMWARP with an optimization-based untangler.Comment: Revision of earlier version of paper. Submitted for publication in
BIT Numerical Mathematics on 27 April 2010. Accepted for publication on 7
September 2010. Published online on 9 October 2010. The final publication is
available at http://www.springerlink.co
Co-production of hydrogen and ethanol from glucose in Escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd)
Background: Biologically, hydrogen (H-2) can be produced through dark fermentation and photofermentation. Dark fermentation is fast in rate and simple in reactor design, but H-2 production yield is unsatisfactorily low as < 4 mol H-2/ mol glucose. To address this challenge, simultaneous production of H-2 and ethanol has been suggested. Co-production of ethanol andH(2) requires enhanced formation of NAD(P) H during catabolism of glucose, which can be accomplished by diversion of glycolytic flux from the Embden-Meyerh-of-Parnas (EMP) pathway to the pentose-phosphate (PP) pathway in Escherichia coli. However, the disruption of pgi (phosphoglucose isomerase) for complete diversion of carbon flux to the PP pathway made E. coli unable to grow on glucose under anaerobic condition. Results: Here, we demonstrate that, when glucose-6-phosphate dehydrogenase (Zwf) and 6-phosphogluconate dehydrogenase (Gnd), two major enzymes of the PP pathway, are homologously overexpressed, E. coli.pgi can recover its anaerobic growth capability on glucose. Further, with additional deletions of Delta hycA,Delta hyaAB,Delta hybBC,Delta ldhA, and Delta frdAB, the recombinant.pgi mutant could produce 1.69 mol H-2 and 1.50 mol ethanol from 1 mol glucose. However, acetate was produced at 0.18 mol mol(-1) glucose, indicating that some carbon is metabolized through the Entner-Doudoroff (ED) pathway. To further improve the flux via the PP pathway, heterologous zwf and gnd from Leuconostoc mesenteroides and Gluconobacter oxydans, respectively, which are less inhibited by NADPH, were overexpressed. The new recombinant produced more ethanol at 1.62 mol mol(-1) glucose along with 1.74 mol H-2 mol(-1) glucose, which are close to the theoretically maximal yields, 1.67 mol mol(-1) each for ethanol andH(2). However, the attempt to delete the ED pathway in the.pgi mutant to operate the PP pathway as the sole glycolytic route, was unsuccessful. Conclusions: By deletion of pgi and overexpression of heterologous zwf and gnd in E. coli Delta hycA Delta hyaAB Delta hybBC Delta ldhA Delta frdAB, two important biofuels, ethanol andH(2), could be successfully co-produced at high yields close to their theoretical maximums. The strains developed in this study should be applicable for the production of other biofuels and biochemicals, which requires supply of excessive reducing power under anaerobic conditions
New insights into electron spin dynamics in the presence of correlated noise
The changes of the spin depolarization length in zinc-blende semiconductors
when an external component of correlated noise is added to a static driving
electric field are analyzed for different values of field strength, noise
amplitude and correlation time. Electron dynamics is simulated by a Monte Carlo
procedure which keeps into account all the possible scattering phenomena of the
hot electrons in the medium and includes the evolution of spin polarization.
Spin depolarization is studied by examinating the decay of the initial spin
polarization of the conduction electrons through the D'yakonov-Perel process,
the only relevant relaxation mechanism in III-V crystals. Our results show
that, for electric field amplitude lower than the Gunn field, the dephasing
length shortens with the increasing of the noise intensity. Moreover, a
nonmonotonic behavior of spin depolarization length with the noise correlation
time is found, characterized by a maximum variation for values of noise
correlation time comparable with the dephasing time. Instead, in high field
conditions, we find that, critically depending on the noise correlation time,
external fluctuations can positively affect the relaxation length. The
influence of the inclusion of the electron-electron scattering mechanism is
also shown and discussed.Comment: Published on "Journal of Physics: Condensed Matter" as "Fast Track
Communications", 11 pages, 9 figure
Negative Thermal Expansion Coefficient of Graphene Measured by Raman Spectroscopy
The thermal expansion coefficient (TEC) of single-layer graphene is estimated
with temperature-dependent Raman spectroscopy in the temperature range between
200 and 400 K. It is found to be strongly dependent on temperature but remains
negative in the whole temperature range, with a room temperature value of
-8.0x10^{-6} K^{-1}. The strain caused by the TEC mismatch between graphene and
the substrate plays a crucial role in determining the physical properties of
graphene, and hence its effect must be accounted for in the interpretation of
experimental data taken at cryogenic or elevated temperatures.Comment: 17 pagese, 3 figures, and supporting information (4 pages, 3
figures); Nano Letters, 201
Adenovirus Encoding Human Platelet-Derived Growth Factor-B Delivered to Alveolar Bone Defects Exhibits Safety and Biodistribution Profiles Favorable for Clinical Use
Abstract Platelet-derived growth factor (PDGF) gene therapy offers promise for tissue engineering of tooth-supporting alveolar bone defects. To date, limited information exists regarding the safety profile and systemic biodistribution of PDGF gene therapy vectors when delivered locally to periodontal osseous defects. The aim of this preclinical study was to determine the safety profile of adenovirus encoding the PDGF-B gene (AdPDGF-B) delivered in a collagen matrix to periodontal lesions. Standardized alveolar bone defects were created in rats, followed by delivery of matrix alone or containing AdPDGF-B at 5.5-108 or 5.5-109 plaque-forming units/ml. The regenerative response was confirmed histologically. Gross clinical observations, hematology, and blood chemistries were monitored to evaluate systemic involvement. Bioluminescence and quantitative polymerase chain reaction were used to assess vector biodistribution. No significant histopathological changes were noted during the investigation. Minor alterations in specific hematological and blood chemistries were seen; however, most parameters were within the normal range for all groups. Bioluminescence analysis revealed vector distribution at the axillary lymph nodes during the first 2 weeks with subsequent return to baseline levels. AdPDGF-B was well contained within the localized osseous defect area without viremia or distant organ involvement. These results indicate that AdPDGF-B delivered in a collagen matrix exhibits acceptable safety profiles for possible use in human clinical studies.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78106/1/hum.2008.114.pd
Application of Graphene within Optoelectronic Devices and Transistors
Scientists are always yearning for new and exciting ways to unlock graphene's
true potential. However, recent reports suggest this two-dimensional material
may harbor some unique properties, making it a viable candidate for use in
optoelectronic and semiconducting devices. Whereas on one hand, graphene is
highly transparent due to its atomic thickness, the material does exhibit a
strong interaction with photons. This has clear advantages over existing
materials used in photonic devices such as Indium-based compounds. Moreover,
the material can be used to 'trap' light and alter the incident wavelength,
forming the basis of the plasmonic devices. We also highlight upon graphene's
nonlinear optical response to an applied electric field, and the phenomenon of
saturable absorption. Within the context of logical devices, graphene has no
discernible band-gap. Therefore, generating one will be of utmost importance.
Amongst many others, some existing methods to open this band-gap include
chemical doping, deformation of the honeycomb structure, or the use of carbon
nanotubes (CNTs). We shall also discuss various designs of transistors,
including those which incorporate CNTs, and others which exploit the idea of
quantum tunneling. A key advantage of the CNT transistor is that ballistic
transport occurs throughout the CNT channel, with short channel effects being
minimized. We shall also discuss recent developments of the graphene tunneling
transistor, with emphasis being placed upon its operational mechanism. Finally,
we provide perspective for incorporating graphene within high frequency
devices, which do not require a pre-defined band-gap.Comment: Due to be published in "Current Topics in Applied Spectroscopy and
the Science of Nanomaterials" - Springer (Fall 2014). (17 pages, 19 figures
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